Objective: This mixed-methods study explored providers’ perceptions of the value of using pharmacogenetic (PGx) testing in depression treatment after they had used the test during a pragmatic clinical trial. Methods: Data were drawn from baseline and follow-up surveys (mental health and primary care providers; N=217) of and qualitative interviews (N=61) with trial participants. Pre-post changes in agreement with statements about PGx testing’s value in depression care were examined with a generalized estimating equations cumulative logit ordinal regression model with two time points and robust standard errors. Interviews were analyzed via rapid qualitative analysis. Results: Analyses showed greater agreement at follow-up with statements about comfort with PGx testing, feeling well informed about PGx testing, and the strength of the evidence base. Mental health providers had stronger levels of agreement compared with primary care providers. Interview data indicated that although provider