A targetable epigenetic vulnerability in PI3K/AKT inhibitor resistant cancers

Acquisition of resistance to PI3K/AKT-targeted monotherapy implies the existence of common resistance mechanisms independent of cancer type. Here we demonstrate that PI3K/AKT inhibitors cause glycolytic crisis, acetyl-CoA shortage and a global decrease in histone acetylation. Also, PI3K/AKT inhibitors induce drug resistance by selectively augmenting H3K27 acetylation and binding of CBP/p300 and…

Prostate Epithelial Genes Define Docetaxel-Sensitive Prostate Cancer Molecular Subtype

BACKGROUND & OBJECTIVES Analysis of the transcriptomic landscape of prostate adenocarcinoma shows multidimensional gene expression variability. Understanding cancer transcriptome complexity can provide biological insight and therapeutic guidance. To avoid potential confounding factors, such as stromal contamination and stress-related material degradation, we utilized a set of genes expressed by…

European agroforestry is no universal remedy for biodiversity: a time-cumulative meta-analysis

Background: Agroforestry is a production system combining trees with crops or livestock. It has the potential to increase biodiversity in relation to single-use systems, such as pastures or conventional agriculture, by providing a higher habitat heterogeneity. In a literature review and subsequent meta-analysis, we investigated the relationship between biodiversity and agroforestry and critically…

A glycoengineered antigen exploiting a conserved protein O-glycosylation pathway in the Burkholderia genus for diagnosis of glanders infections

We recently described a protein O-glycosylation pathway conserved in all species of the Burkholderia genus that results in synthesis and incorporation of a trisaccharide glycan to membrane-exported proteins. Here, we exploited this system to construct and evaluate a diagnostic tool for glanders. Burkholderia mallei, the causative agent of glanders, is a highly infectious and fatal zoonotic…

Feasibility of phosphoproteomics on leftover samples after RNA extraction with guanidinium thiocyanate

In daily practice, different types of biomolecules are usually extracted for large-scale “omics” analysis with tailored protocols. However, when sample material is limited, an all-in-one strategy is preferable. While lysis of cells and tissues with urea is the accepted standard for phosphoproteomic applications, DNA, RNA and proteins can be simultaneously extracted from small samples using acid…

Experimental pieces of evidence for Mycobacterium ulcerans dormancy.

Background Whether Mycobacterium ulcerans, the etiological agent of the neglected Buruli ulcer in numerous tropical countries, would exist in a dormant state as reported for closely related Mycobacterium species, is not established. Methodology Six M. ulcerans strains were exposed to a progressive depletion in oxygen for two months, using a previously described Wayne model of dormancy; and…

SARS-CoV-2 lineage B.6 is the major contributor to transmission in Malaysia

Background: As of June 30, 2020, Malaysia had confirmed 8,639 cases of COVID-19. About 39% of these were associated with a religious mass gathering event held in Kuala Lumpur between February 27 and March 1, 2020, which drove community transmission during Malaysia’s main wave. We analysed genome sequences of SARS-CoV-2 from Malaysia to understand the molecular epidemiology. Methods: We obtained…

Phylogenetic population structure  and  drug resistance of Mycobacterium tuberculosis complex in the Volta Region of Ghana

Context: Available molecular epidemiological data from recent studies suggest significant genetic variation between the different phylogenetic lineages of  Mycobacterium tuberculosis complex (MTBC ) and the MTBC lineages might have adapted to different human populations   Aim: This study sought to determine the phylogenetic population structure of clinical MTBC isolates from the Volta Region of…

A comparison of several media types and basic techniques used to assess outdoor airborne fungi in Melbourne, Australia.

Despite the recent increase in interest in indoor air quality regarding mould, t here is no single widely accepted standard media for the detection of airborne fungi, nor verification of many commonly used techniques. Commonly used media including malt-extract agar (MEA), Sabouraud dextrose agar (Sab, SDA, SabCG), potato dextrose agar (PDA) with and without antibiotics chloramphenicol &…

Epidemiology of Dengue viral infection in Nepal: 2006-2019

Background Dengue is one of the newest emerging diseases in Nepal with increasing burden and geographic spread over the last 14 years. The main objective of this study was to explore the spatio-temporal epidemiological patterns of Dengue since its first report (2006) till 2019 in Nepal. Methods This study is a retrospective analysis of dengue data available from the Epidemiological Disease…