This study protocol describes a systematic method to identify, collect and rank sports science and medicine interventions most associated with optimising team performance in elite football in observational studies. While numerous interventions, such as conditioning or injury prevention programmes, protective equipment, training periodisation, tactical decision-making, supplements, medication and hydration administration, have been associated with football players and team performance enhancement, there is a need to prioritise them to save resources and increase the efficiency of applications. Nevertheless, previous literature has shown that systematic reviews in elite football often need more protocol registration and have limited procedures, synthesis and practical implications directly applicable to the field. Therefore, this protocol outlines a comprehensive process developed following the Cochrane Collaboration and Preferred Reporting Items for Systematic Reviews and Meta-Analyses