• Mashup Score: 3

    Genome engineering of bacteriophages provides opportunities for precise genetic dissection and for numerous phage applications including therapy. However, few methods are available for facile construction of unmarked precise deletions, insertions, gene replacements and point mutations in bacteriophages for most bacterial hosts. Here we describe CRISPY-BRED and CRISPY-BRIP, methods for efficient…

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  • Mashup Score: 10

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  • Mashup Score: 20

    Phage therapy, which is the use of bacterial viruses called bacteriophages to treat bacterial infections, has been studied since 1919, with the Eliava Institute in Tbilisi, Georgia, playing a leading role during much of that time, but also with substantial use of bacteriophages in both Europe and North America.1 Interest in phage therapy has slowly diminished in favour of antibiotics since about…

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